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Um and Tissue Samples for Patient's with Biopsy Proven or Suspected Malignant Disease. Immediately after surgical resection tissues were snap frozen and kept at -80 until RNA isolation. For RT-PCR assessment of TUSC2 expression we used tumor/control matched specimens derived from thirty MPM patients. Control specimens were derived from unaffected peritoneum. RNA from these specimens was extracted
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Nstitute (Detroit, MI). They contained a total of 30 cases represented by 2-mm cores of resected tumors and adjacent mesothelial tissues. A validating set of four TMA slides containing 75 MPM cases from the MD Anderson Cancer Center (Houston, TX) were constructed using triplicate 1 mm diameter cores from each tumor. All patients gave informed consent for de-identified use of tissues. Both studies
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